if you need to read the values from the last line, then
DataRow lastRow = yourTable.Rows[yourTable.Rows.Count-1];
will return you the last line. and you can read the values from it.
My second assumption is that by datatable you are referencing a table in sql server.
Then with a little modification, your request is also fine.
SELECT TOP 1 COLUMN FROM TABLE ORDER BY COLUMN DESC
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