A pixel is a relative unit of measure; it does not have an absolute size.
Edit Have . As for your editing: again, you can calculate the distance between two pixels in an image in pixels, not centimeters. As a simple example, think about video projectors: if you project, say, a 3 × 3px image onto a wall, the distance between the leftmost and rightmost pixels can range from a few millimeters to several meters. If you move the projector closer to or further from the wall, the pixel size will change and any distance that you calculated earlier will become incorrect.
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