Of course, this is possible in theory. But if your file system is NTFS, be prepared to spend several months examining all the data structures that you will need to update. (All of them are officially undocumented by BTW.)
In addition, you will need
- Somehow turn off the volume and make changes; or
- Learn how to write a kernel file system driver, buy a license from MS, develop a driver and use it to make changes to a live file system.
It is a little easier if your file system is simpler than FAT32. But in any case: in short, it is possible, but even if it takes years from your life. My advice: do not worry.
: . avisynth script, .