You can read the entire file, and then the ziplines to transpose them, and then combine the result to smooth the list. Standalone example (using a list of strings as input):
import csv,itertools
text="""a,b,c
1,2,3
4,5,6
7,8,9
""".splitlines()
myList = list(itertools.chain.from_iterable(zip(*csv.reader(text[1:]))))
print(myList)
result:
['1', '4', '7', '2', '5', '8', '3', '6', '9']
, :
with open("test.csv") as f:
cr = csv.reader(f,separator=",")
next(cr)
myList = list(itertools.chain.from_iterable(zip(*cr)))