, , :
ggplot(data = dat, aes(x = Row, y = Col)) +
geom_tile(aes(fill = Y1), colour = "white") +
scale_fill_brewer(palette = "PRGn")+
geom_point(data = dat, aes(size="NA"), shape =NA, colour = "grey95")+
guides(size=guide_legend("NA", override.aes=list(shape=15, size = 10)))

NA:
ggplot(data = dat, aes(x = Row, y = Col)) +
geom_tile(aes(fill = Y1), colour = "white") +
scale_fill_brewer(palette = "PRGn", na.value="red")+
geom_point(data = dat, aes(size="NA"), shape =NA, colour = "red")+
guides(size=guide_legend("NA", override.aes=list(shape=15, size = 10)))
