If you use a website that is associated with a web application project, you probably do not have the actual solution or even the project file.
For a website, you need to rebuild the website (from the build menu) or manually remove all the DLLs to accomplish the same thing.
Edit:
How do you debug? Are you using Cassini (the built-in visual studio debugger) or are you connecting to the IIS process?
I will also try:
Go to the folder C: \ WINDOWS \ Microsoft.NET \ Framework \ v2.0.50727 \ Temporary ASP.NET and delete the folder with the same name as your website.
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