Of course, you will need to install the .NET Framework on your server. If you use the AJAX Toolkit, you will want to copy the AjaxControlToolkit.dll file to the bin folder of your web application on your server.
Also, make sure you configure your web application to use the .NET Framework 2.0. In IIS, you go to the properties of your website, and then on the ASP.NET tab and make sure that the version of ASP.NET is set to 2.0.
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